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human fetal lung fibroblasts mrc5  (ATCC)


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    ATCC human fetal lung fibroblasts mrc5
    Human Fetal Lung Fibroblasts Mrc5, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fetal+lung+fibroblasts+mrc5/MRC-5%3B+Lung+Fibroblast%3B+Human/pm41224821-251-0-6
    Average 97 stars, based on 1574 article reviews
    human fetal lung fibroblasts mrc5 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Differentiation and Growth-Arrest-Related lncRNA ( DAGAR ): Initial Characterization in Human Smooth Muscle and Fibroblast Cells
    Article Snippet: Medium was changed every 48 h. Human pulmonary artery endothelial cells (hPAECs, Lonza Group, Switzerland) were cultured according to manufacturer’s instructions and supplemented with 10% FBS (Gibco, Thermo Fisher, USA). .. Human embryonic kidney 293 cells (ATCC, Manassas, VA, USA) and human fetal lung fibroblasts MRC5 (ATCC, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Merck KGaA, St. Louis, MO, USA) supplemented with 10% FBS (Gibco, Thermo Fisher, USA) and 1% Penicillin/Streptomycin antibiotic mix (Sigma-Aldrich, USA). ..

    Article Title: Differentiation and Growth-Arrest-Related lncRNA ( DAGAR ): Initial Characterization in Human Smooth Muscle and Fibroblast Cells.
    Article Snippet: Medium was changed every 48 h. Human pulmonary artery endothelial cells (hPAECs, Lonza Group, Switzerland) were cultured according to manufacturer’s instructions and supplemented with 10% FBS (Gibco, Thermo Fisher, USA). .. Human embryonic kidney 293 cells (ATCC, Manassas, VA, USA) and human fetal lung fibroblasts MRC5 (ATCC, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Merck KGaA, St. Louis, MO, USA) supplemented with 10% FBS (Gibco, Thermo Fisher, USA) and 1% Penicillin/Streptomycin antibiotic mix (Sigma-Aldrich, USA). ..

    Article Title: Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo
    Article Snippet: .. Human carcinoma cell line RKO (ATCC CRL-2577), human fetal lung fibroblasts MRC5 transformed with SV-40 (MRC5 SV2, EcACC Cat. No. 84100401), and human endothelial cell line EA.hy926 (ATCC CRL-2922) were cultured in DMEM medium (Dulbecco's modified Eagle's medium) (Gibco, USA) supplemented with 2 mM glutamine and 10% fetal bovine serum (FBS) (HyClone, USA) and 100 U/ml streptomycin and 100 U/ml penicillin (all from Gibco, CA). .. HeLa cells were cultured in minimal essential medium with Earle's salts (MEM, PAA Lab GmbH, E15–888) with 5.6 mM glucose, 2 mM stable glutamine, and sodium bicarbonate, supplemented with 10% FBS (Biochrom AG), 1% MEM nonessential amino acids (Biochrom AG), and 1% 4−(2−hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, PAA Lab GmbH).

    Article Title: Expression of the Calcium-Binding Protein CALB1 Is Induced and Controls Intracellular Ca 2+ Levels in Senescent Cells
    Article Snippet: Human mammary epithelial cells (HMEC, CC-2551B, Lonza, Basel, Switzerland) were cultured in a mammary epithelial cell growth medium (C-21210, Promocell, Heidelberg, Germany) supplemented with 1% penicillin/streptomycin (Life Technologies, Carlsbad, CA, USA). .. Human fetal lung fibroblasts MRC5 (ATCC), 293 GP retrovirus-producing cells, and 293 T lentivirus-producing cells (Clontech, Mountain View, CA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM supplemented in GlutaMax, Life Technologies) with 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (ThermoFisher Scientific, Waltham, MA, USA). .. All the cells were cultured at 37 °C with 5% CO 2 . (Z)-4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich) was used at 100 nM, bleomycin (Merck, Kenilworth, NJ, USA) at 12 μg/mL, nutlin-3 (Sigma-Aldrich) at 1 μM, H 2 O 2 (Sigma) at 250 μM, TGFβ (Peprotech, Rocky Hill, NJ, USA) at 0.5 ng/mL, KCl (Sigma-Aldrich) at 65 mM and FK-506 (MedChem Express, Monmouth Junction, NJ, USA) at 2 μM.

    Modification:

    Article Title: Differentiation and Growth-Arrest-Related lncRNA ( DAGAR ): Initial Characterization in Human Smooth Muscle and Fibroblast Cells
    Article Snippet: Medium was changed every 48 h. Human pulmonary artery endothelial cells (hPAECs, Lonza Group, Switzerland) were cultured according to manufacturer’s instructions and supplemented with 10% FBS (Gibco, Thermo Fisher, USA). .. Human embryonic kidney 293 cells (ATCC, Manassas, VA, USA) and human fetal lung fibroblasts MRC5 (ATCC, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Merck KGaA, St. Louis, MO, USA) supplemented with 10% FBS (Gibco, Thermo Fisher, USA) and 1% Penicillin/Streptomycin antibiotic mix (Sigma-Aldrich, USA). ..

    Article Title: Differentiation and Growth-Arrest-Related lncRNA ( DAGAR ): Initial Characterization in Human Smooth Muscle and Fibroblast Cells.
    Article Snippet: Medium was changed every 48 h. Human pulmonary artery endothelial cells (hPAECs, Lonza Group, Switzerland) were cultured according to manufacturer’s instructions and supplemented with 10% FBS (Gibco, Thermo Fisher, USA). .. Human embryonic kidney 293 cells (ATCC, Manassas, VA, USA) and human fetal lung fibroblasts MRC5 (ATCC, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Merck KGaA, St. Louis, MO, USA) supplemented with 10% FBS (Gibco, Thermo Fisher, USA) and 1% Penicillin/Streptomycin antibiotic mix (Sigma-Aldrich, USA). ..

    Article Title: Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo
    Article Snippet: .. Human carcinoma cell line RKO (ATCC CRL-2577), human fetal lung fibroblasts MRC5 transformed with SV-40 (MRC5 SV2, EcACC Cat. No. 84100401), and human endothelial cell line EA.hy926 (ATCC CRL-2922) were cultured in DMEM medium (Dulbecco's modified Eagle's medium) (Gibco, USA) supplemented with 2 mM glutamine and 10% fetal bovine serum (FBS) (HyClone, USA) and 100 U/ml streptomycin and 100 U/ml penicillin (all from Gibco, CA). .. HeLa cells were cultured in minimal essential medium with Earle's salts (MEM, PAA Lab GmbH, E15–888) with 5.6 mM glucose, 2 mM stable glutamine, and sodium bicarbonate, supplemented with 10% FBS (Biochrom AG), 1% MEM nonessential amino acids (Biochrom AG), and 1% 4−(2−hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, PAA Lab GmbH).

    Article Title: Expression of the Calcium-Binding Protein CALB1 Is Induced and Controls Intracellular Ca 2+ Levels in Senescent Cells
    Article Snippet: Human mammary epithelial cells (HMEC, CC-2551B, Lonza, Basel, Switzerland) were cultured in a mammary epithelial cell growth medium (C-21210, Promocell, Heidelberg, Germany) supplemented with 1% penicillin/streptomycin (Life Technologies, Carlsbad, CA, USA). .. Human fetal lung fibroblasts MRC5 (ATCC), 293 GP retrovirus-producing cells, and 293 T lentivirus-producing cells (Clontech, Mountain View, CA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM supplemented in GlutaMax, Life Technologies) with 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (ThermoFisher Scientific, Waltham, MA, USA). .. All the cells were cultured at 37 °C with 5% CO 2 . (Z)-4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich) was used at 100 nM, bleomycin (Merck, Kenilworth, NJ, USA) at 12 μg/mL, nutlin-3 (Sigma-Aldrich) at 1 μM, H 2 O 2 (Sigma) at 250 μM, TGFβ (Peprotech, Rocky Hill, NJ, USA) at 0.5 ng/mL, KCl (Sigma-Aldrich) at 65 mM and FK-506 (MedChem Express, Monmouth Junction, NJ, USA) at 2 μM.

    Transformation Assay:

    Article Title: Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo
    Article Snippet: .. Human carcinoma cell line RKO (ATCC CRL-2577), human fetal lung fibroblasts MRC5 transformed with SV-40 (MRC5 SV2, EcACC Cat. No. 84100401), and human endothelial cell line EA.hy926 (ATCC CRL-2922) were cultured in DMEM medium (Dulbecco's modified Eagle's medium) (Gibco, USA) supplemented with 2 mM glutamine and 10% fetal bovine serum (FBS) (HyClone, USA) and 100 U/ml streptomycin and 100 U/ml penicillin (all from Gibco, CA). .. HeLa cells were cultured in minimal essential medium with Earle's salts (MEM, PAA Lab GmbH, E15–888) with 5.6 mM glucose, 2 mM stable glutamine, and sodium bicarbonate, supplemented with 10% FBS (Biochrom AG), 1% MEM nonessential amino acids (Biochrom AG), and 1% 4−(2−hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, PAA Lab GmbH).



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